COLIPLATE™- 400

INTENDED USE The ColiPlate™-400 is a rapid, convenient and accurate test for quantitative measure of total coliforms and E. coli. The test is designed to meet regulatory guidelines for surface water, recreational water, processing water and wastewater. The ColiPlate enables quantification of coliforms and E. coli density ranging between ca. 3 to 2,400 cfu/ 100 ml in a single test unit without dilution. The distinctive blue/green coloration of positive tested samples enables analysis of brownish, turbid or rust filled waters.

TEST PRINCIPLE AND FEATURES The ColiPlate test utilizes the proven X-Gal and MUG techniques to detect viable coliforms and/or E. coli bacteria. The ColiPlate contains selective media to provide nutrients to stimulate the growth of coliforms and E. coli. The media also contains inducers and chromogenic/fluorogenic substrates. These substrates react with specific enzyme indicative of coliforms (beta-D-galactosidase) and E. coli (beta-D-glucoronidase) to provide color change to blue/green and fluorescence by coliforms and E. coli respectively. Test results are recorded after just 24 hours of incubation with the appearance of blue/green color for coliforms. E. coli can be detected by the appearance of fluorescence under a long wavelength UV light. Quantification is based on Most Probable Number (MPN) of colony forming-units (cfu) per 100 ml sample.

BASED ON – DIRECT POUR METHOD